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Vision Science Seminar

 

UPenn - Vision Research Center Nikon A1R Confocal Microscope

UPenn - School of Dental Medicine Center for Cellular Imaging

Quick Fact Sheet

 

Location:

 

514 Levy Building

 

New users, contact:

Anu Dhingra 513 Levy dhingra@mail.med.upenn.edu

215 746 0022

Kathy Boesze Battaglia, PhD 515 Levy battagli@dental.upenn.edu 215 898 9167

 

Calender url:

https://www.google.com/calendar/embed?src=sdm.imaging@gmail.com

 

Nikon A1R Confocal Microscope

  • Transmitted light, DIC and epifluorescence (Dapi, FITC, TRITC filters)
  • Objectives: 20x Dry, 40x Dry, 60x water, 100x oil
  • Fully automatated XYZ scanning stage
    • Piezo Z stage insert for high speed image capture when using resonance scanner.
    • Perfect Focus System – allowing for accurate stage reposition over timed series events.
  • Environmental chamber
    • Adjustable CO2 percentage and temperature with constant humidity.
    • Accommodates 35 mm glass bottom culture dishes, 30x60mm chamber slides and 25x75mm glass slides.
  • Scan Heads for Image capture – Galvo, up to 4 fps (512x512)
    • Resonance 30 fps (512x512), up tp 230 fps (512x64)
  • Stage:
    • Fully automated XYZ scanning. 
    • Piezo Z stage insert for high speed image capture with resonance scanner.
    • Perfect Focus System – allowing accurate stage reposition over a time series.
  • 4 Laser lines with filter configuration providing the following:
Excitation wavelengths (nm)                            Emission Filters
405 425-475
457 465-505
488 500-550
561 575-625
638 650 Long Pass

Detectors

  • 4- Dedicated PMT for laser lines
  • 1-DIC PMT
  • DUS Spectral detector- 32 channels of detection over the 400nm-750mn.  Spectral resolution adjusted to 2.5nm, 6nm and 10nm channel width    

Image Analysis

  • Nikon NIS Elemtents software
  • Additional Elements Software Workstation
  • SDM server access
  • Secure Share