UPenn - Vision Research Center Molecular Biology Example Projects
June 2012: Effect of Oxidative Stress in Cultured Cells and in Mice
Joshua Dunaief and colleagues used the VRC real time PCR machines in his study of the role of Bmp6 on the regulation of retinal iron homeostasis in age related macular degeneration. The figure below (from Hadziahmetovic et al., 2011) shows the effect of oxidative stress in cultured cells and in mice on Bmp6 and Hmox1 mRNAs assessed by qPCR.
Effect of oxidative stress in cultured cells and in mice on Bmp6 and Hmox1 mRNAs assessed by qPCR. Sixteen hours of treatment of ARPE-19 cells with 75?M H2O2 significantly upregulated Hmox1 (A) while down-regulating Bmp6 mRNA (B) relative to untreated controls (MEM only; n=6). Following photic injury Hmox1 mRNA levels were significantly increased within isolated RPE (C) while Bmp6 mRNA levels were significantly decreased (D) compared to control. * p<0.05
Reference: Hadziahmetovic M, Song Y, Wolkow N, Iacovelli J, Kautz L, Roth MP, Dunaief JL. Bmp6 regulates retinal iron homeostasis and has altered expression in age-related macular degeneration. Am J Pathol. 2011 Jul;179(1):335-48. Epub 2011 May 3. PubMed PMID: 21703414; PubMed Central PMCID: PMC3123855.